Search results for "manganese chloride"

showing 4 items of 4 documents

Manganese Ions Individually Alter the Reverse Transcription Signature of Modified Ribonucleosides

2020

Reverse transcription of RNA templates containing modified ribonucleosides transfers modification-related information as misincorporations, arrest or nucleotide skipping events to the newly synthesized cDNA strand. The frequency and proportion of these events, merged from all sequenced cDNAs, yield a so-called RT signature, characteristic for the respective RNA modification and reverse transcriptase (RT). While known for DNA polymerases in so-called error-prone PCR, testing of four different RTs by replacing Mg2+ with Mn2+ in reaction buffer revealed the immense influence of manganese chloride on derived RT signatures, with arrest rates on m1A positions dropping from 82% down to 24%. Additi…

0301 basic medicinelcsh:QH426-470DNA polymerasechemistry.chemical_elementManganeseSaccharomyces cerevisiaeRT signature[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology01 natural sciencesArticle03 medical and health sciencesm1ARNA modificationsComplementary DNA[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]GeneticsNucleotidem<sup>1</sup>ABase PairingGenetics (clinical)PolymeraseComputingMilieux_MISCELLANEOUSchemistry.chemical_classificationIonsManganesebiology010405 organic chemistryRNARNA-Directed DNA Polymerase[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyreverse transcriptionMolecular biologyReverse transcriptase0104 chemical scienceslcsh:Genetics030104 developmental biologyTemplatechemistrybiology.proteinRNA[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]Ribonucleosidesmanganese chloride
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CCDC 869843: Experimental Crystal Structure Determination

2013

Related Article: E.Pardo, C.Train, K.Boubekeur, G.Gontard, J.Cano, F.Lloret, K.Nakatani, M.Verdaguer|2012|Inorg.Chem.|51|11582|doi:10.1021/ic3014915

Space GroupCrystallographyCrystal SystemCrystal StructureCell Parameterscatena-(tetrakis(4-NN-dimethylaminopyridinium) bis(mu~2~-oxalato)-aqua-chloro-(oxalato)-chromium-manganese bis(mu~2~-oxalato)-diaqua-(oxalato)-chromium-manganese chloride ethanol solvate monohydrate)Experimental 3D Coordinates
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CCDC 900041: Experimental Crystal Structure Determination

2013

Related Article: M. Clemente-Leon, E. Coronado, M. Lopez-Jorda|2013|Eur.J.Inorg.Chem.||753|doi:10.1002/ejic.201201113

Space GroupCrystallographycatena-[tris(bis(22'-(25811-Tetraazadodeca-111-diene-112-diyl)bis(5-bromophenolato))-iron) hexakis(mu~2~-oxalato)-di-chromium-di-manganese chloride methanol solvate]Crystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 945720: Experimental Crystal Structure Determination

2013

Related Article: N. Marino, D. Armentano, T. F. Mastropietro, M. Julve, G. De Munno and J. Martinez-Lillo|2013|Inorg.Chem.|52|11934|doi:10.1021/ic4016355

Space GroupCrystallographytetrakis(mu~3~-5-(hydroxymethyl)-2-methyl-4-(oxidomethyl)pyridinium-3-olato)-tris(mu~2~-acetato)-dichloro-tetra-manganese chloride methanol solvate dihydrateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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